How to use NCBI database ---->sequence-->gene-->disease

I.Entrez Sequence Search

“Phenylalanine Hydroxylase” AND human[orgn] AND biomol_mrna[properties] NOT gbdiv_est[properties]

可以查到一段sequence

之後進行比對

Copy the Go to www.ncbi.nih.gov/BLAST/
Select “nucleotide blast” (blastn)

II: BLAST: mapping primer to the human genome

Upstream primer     5´-CCATGGCGACCCTGGAAAAGC-3´

Downstream primer 5 ´-CAGCAGCGGCTGTGCCTGCGG-3 ´

http://www.ncbi.nlm.nih.gov/genome/seq/BlastGen/BlastGen.cgi?taxid=9606

CCATGGCGACCCTGGAAAAGC

NNNNNNNNNNNNNNNNNNNNN

CAGCAGCGGCTGTGCCTGCGG

補N後 貼到BLAST的地方

Adjustments needed:
- Paste in converted primers
- Change database to: genome (reference only)
- Change program to blastn
- Increase Expect to 10
- Change filter to “None”
- Hit submit to search

之後就會得到結果

III.BLAST : sample domain search

http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi

貼上sequence後比對